INVESTIGATIONS FOR DETERMINATION OF TANNING AGENTS IN SEA BUCKTHORN (HIPPOHAE RHAMNOIDES) FRUITS

Download full text PDF
DOI: https://doi.org/None
Issue: 
3
Year: 
2016

O.V. Trineeva, PhD; N.S. Shikunova; Professor A.I. Slivkin, PhD Voronezh State University; 1, Universtitetskaya Square, Voronezh 394006, Russia

Sea buckthorn (Hippophae rhamnoides L.) is a valuable medicinal plant. The State Pharmacopeia contains no articles on its fruits. Different procedures for determination of tanning agents in the sea buckthorn fruits were comparatively analyzed to work out a current normative document. The spectrophometric determination procedure, the basis for which is that the optic density of hydroalcoholic extraction from the fruits is measured at a wavelength of 275±2 nm, is most preferable. The mean determination error is about 2%, which is 3-5 times lower than that in the application of other procedures. A procedure was developed and validated for quantification of tanning agents in the sea buckthorn fruits, calculated with reference to tannin. Preservation was investigated for is impact on the stability of tanning agents in the fruits. Dried ground fruits are recommended as a source of a complex of polyphenolic compounds.
Keywords: 
sea buckthorn
Hippophae rhamnoides L.
fresh
frozen fruits
tanning agents
determination
References: 
  1. Gosudarstvennaya farmakopeya Rossiyskoy Federacii XIII izd. OFS «Opredelenie dubil`nyh veshhestv v lekarstvennom rastitel`nom syr`e». (http://www.rosminzdrav.ru/ministry/61/11/ materialy-po-deyatelnosti-deparatamenta/stranitsa-856/spisokobschih- farmakopeynyh-statey). (data obrashheniya 11.10.2015).
  2. Danilova N.A., Popov D.M. Kolichestvennoe opredelenie dubil`nyh veshhestv v kornyah shhavelya konskogo metodom spektrofotometrii v sravnenii s metodom permanganatometrii. Vestnik VGU. Ser.: Himiya, Biologiya, Farmaciya, 2004; 2: 179–182.
  3. Grin`ko E.N. Trebovaniya Rossiyskoy i Evropeyskoy farmakopey k metodikam opredeleniya soderzhaniya dubil`nyh veshhestv v lekarstvennom rastitel`nom syr`e. Farmaciya, 2010; 5: 49–53.
  4. GOST SSSR 4565-79. List sumaha.
  5. GOST SSSR 4564-79. List skumpii.
  6. Razarenova K.N., Zhohova E.V. Sravnitel`naya ocenka soderzhaniya dubil`nyh veshhestv v nekotoryh vidah roda Geranium L. flory severo-zapada. Himiya rastitel`nogo syr`ya, 2011; 4: 187– 192.
  7. Borovikova N.A., Selezenev N.G., Popov D.M. Spektrofotometricheskoe kolichestvennoe opredelenie dubil`nyh veshhestv v kore duba, soplodiyah ol`hi i v vodnyh izvlecheniyah iz dannogo syr`ya. Voprosy biologicheskoy, medicinskoy i farmacevticheskoy himii, 2010; 11: 19–23.
  8. Mal`ceva A.A., Chistyakova A.S., Sorokina A.A. i dr. Kolichestvennoe opredelenie dubil`nyh veshhestv v trave gorca pochechuynogo. Vestnik VGU. Ser.: Himiya. Biologiya. Farmaciya, 2013; 2: 203–205.
  9. Samylina I.A., Aboyanc R.K., Grin`ko E.N. Sposob opredeleniya dubil`nyh veshhestv v rastitel`nom syr`e. Patent RF 2439568 ot 12.10.2010 g.
  10. Ryabinina E.I., Zotova E.E., Ponomareva N.I. Potenciometricheskoe opredelenie dubil`nyh veshhestv v lekarstvennom rastitel`nom syr`e. Farmaciya, 2012; 8–10.
  11. Sorokina A.A., Marahova A.I. Potenciometriya kak metod kontrolya kachestva lekarstvennogo rastitel`nogo syr`ya, soderzhashhego dubil`nye veshhestva, i preparatov na ego osnove. Farmaciya, 2014; 2: 49–53.
  12. Marahova A.I., Stanishevskiy Ya.M., Potapov V.T., Sorokina A.A. Razrabotka i validaciya metodiki potenciometricheskogo opredeleniya summy dubil`nyh veshhestv v trave zveroboya. Razrabotka i registraciya lekarstvennyh sredstv, 2014; 3 (8): 138– 141.
  13. Abdullina S.G., Agapova N.M., Ziyatdinova G.K. i dr. Kulonometricheskoe opredelenie dubil`nyh veshhestv v lekarstvennom rastitel`nom syr`e. Farmaciya, 2010; 4: 13–15.
  14. Avtory: Korenman Ya.I., Novikova N.A., Niftaliev S.I. Sposob razdel`nogo opredeleniya tannina i katehinov (v pereschete na gallovuyu kislotu) v chae. Patent RF 2127878 ot 20.03.1999 g.
  15. Chumakova V.V., Mezenova T.D., Popova O.I. Opredelenie gallovoy kisloty v trave lofanta anisovogo metodom planarnoy hromatografii. Himiya rastititel`nogo syr`ya, 2011; 4: 269–271.
  16. Popov I.V., Andreeva I.N., Gavrilin M.V. Opredelenie tanina v syr`e i preparatah krovohlebki lekarstvennoy metodom VE`ZhH. Himiko- farmacevticheskiy zhurnal, 2003; 7 (37): 24–26.
  17. Rukovodstvo po metodam kontrolya kachestva i bezopasnosti biologicheski aktivnyh dobavok k pishhe. R 4.1.1672-03. M., 2004: 94–95.