AMINO ACID COMPOSITION OF THE LEAVES AND FRUITS OF CHINESE MAGNOLIA VINE (SCHIZANDRA CHINENSIS) GROWING IN THE VORONEZH REGION

DOI: https://doi.org/None
Issue: 
6
Year: 
2016

Yu.V. Dobrina (1); A.A. Maltseva (1), PhD; Professor A.A. Sorokina (2), PhD; Professor A.I. Slivkin (1), PhD 1 -Voronezh State University; 1, Universitetskaya Sq., Voronezh 394006, Russian Federation 2 -I.M. Sechenov First Moscow State Medical University; 8, Trubetskaya St., Build. 2, Moscow 119991, Russian Federation

Introduction. Medicinal plants are a promising source available for human absorption of amino acids (AA) that can accumulate in free and bound forms in different morphological parts of plants. Chinese magnolia vine is widely used in medical practice. However, reliable information about the AA composition of the morphological parts of the plant is still scarce. Objective: to comparatively investigate the composition of AAs in the Chinese magnolia vine leaves and fruits harvested in the Voronezh Region. Material and methods. The objects of the investigation were dried Chinese magnolia vine leaves and fruits harvested in 2015 from the plants cultivated in the Voronezh Region. The composition of AAs had been previously examined using thin layer chromatography (TLC) on 10х10-cm Sorbfil PTCC-AF-B-UF plates in the butanol-acetic acid-water (4:1:2) system; the detecting reagent was 0.2% alcohol solution of ninhydrin. The amino acid profile of raw Chinese magnolia vine material was investigated in detail by capillary electrophoresis using a Capel – 1055/105 M device. The content of free AAs was measured spectrophotometrically, by applying a SF 2000-01 device at a wavelength of 568 nm. Results. TLC analysis showed that the composition of free AAs in the Chinese magnolia vine leaves varied with the stage of plant growth. The sum of free AAs in the leaves, calculated with reference to glutamic acid, ranged from 0.41% (flowering) to 0.48% (fruiting). The leaves (at the fruiting stage) and fruits of magnolia vine were established to have an equal composition of AAs, but to differ in their quantitative content. The leaves contained almost twice as much as AAs than the fruits. Conclusion. The investigators have identified a specific set of AAs, which is characteristic of the fruits and leaves of magnolia vine at different stages of plant growth. The fruits of magnolia vine and its leaves (during fruiting) contain an equal set of AAs; 17 AAs have been found to be present; 7 of which are essential.

Keywords: 
Chinese magnolia vine
Schizandra chinensis (Turcz.) Baill.
fruits
leaves
amino acids
content
thin layer chromatography
capillary electrophoresis

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