Determination of usnic acid in the blastemas of a lichen of the genus Cladonia by HPLC/UV

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DOI: https://doi.org/10.29296/25419218-2020-03-06
Issue: 
3
Year: 
2020

A.V. Nikulin(1), S.I. Yamshchikova(2), O.G. Potanina(1), R.A. Abramovich(1) 1-Collective Usage Center (Research Educational Center), People’s Friendship University of Russia; 6, Miklukho-Maklai St., Moscow 117198, Russian Federation 2-Medical Institute, M.K. Ammosov North-Eastern Federal University; 27, Oiunsky St., Moscow 677000, Russian Federation

Introduction. Cup moss that is a combination of lichens of the genus Cladonia contains biologically active substances (usnic acids, phenolic compounds, etc.) that have antibacterial and antiviral properties. The sodium salts of usnic acid have long been used in pharmaceutical practice. Various methods (TLC, ELISA, HPLC/MS, etc.) are used to determine usnic acid in plant materials. Cup moss is not an officinal raw material and its estimation by the content of usnic acid is absent. Objective: to develop and validate a procedure for the quantitative determination of usnic acid in the blastemas of the lichen of the genus Cladonia. Material and methods. The investigation object was cup moss samples collected in the Republic of Sakha (Yakutia). Usnic acid was determined by HPLC-UV. Results. A procedure for the quantitative determination of usnic acid in the blastemas of the lichen of the genus Cladonia was developed and validated by HPLC with a measurement error of ±4.47%. Conclusion. The developed method was shown to be promising in analyzing the plant substance for the levels of usnic acid. With its usage, it was found that the content of usnic acid in the blastemas of the cup moss growing in different regions of Yakutia ranges from 0.88 to 1.02%
Keywords: 
usnic acid
blastemas of the lichen of genus Cladonia
quantification
HPLC-UV
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