The Journal is included in Russian and International Library and Abstract Databases
Russain Science Index (Russia)
EBSCO
DOI Registration Agency (USA)
Scientific Indexing Services (USA)
CAS Source index (USA)
Web of Science Group (USA)

Elaboration of approaches to standardizing Emerson’s thorn (Crataegus submollis) fruits

DOI: https://doi.org/10.29296/25419218-2020-06-04
Issue: 
6
Year: 
2020

I.Kh. Shaikhutdinov, V.A. Kurkin, O.E. Pravdivtseva Samara State Medical University, 89, Chapaevskaya St., Samara 443099, Russian Federation

Introduction. The fruits of 12 hawthorn species are currently allowed for the production of drugs used to treat cardiovascular diseases, but not all of them grow widely in the Russian Federation. At the same time, the fruits of Emerson’s thorn (Crataegus submollis Sarg.) that is a widely cultivated North American species are widely used in the food industry, but unused in medical practice. Flavonoids are the leading group of active ingredients in Emerson’s thorn fruits. Objective: to develop a procedure for the quantitative determination of the total flavonoid content in Emerson’s thorn fruits. Materials and methods. The object of the investigation was Emerson’s thorn fruits plucked in the Samara Region in the fall of 2018. Aqueous alcoholic extracts from their raw materials were studied using UV spectrophotometry. Results. A procedure was developed for the quantitative determination of total flavonoid content in Emerson’s thorn fruits by direct spectrophotometry using the state standard sample (SSS) of catechin at an analytical wavelength of 282 nm. The error of a single determination with a 95% confidence level was ±4.44%. Conclusion. The developed procedure was used to determine the total flavonoid content calculated with reference to catechin in Emerson’s thorn fruits, which ranged from 4.17% to 5.35%.

Keywords: 
Emerson’s thorn
Crataegus submollis Sarg
fruits
standardization
flavonoids
catechin
spectrophotometry

References: 
  1. Trees and shrubs of the USSR. Moscow-Leningrad, Edition of the USSR Academy of Sciences, 1954; 3: 872 (In Russian).
  2. Enikeeva K.I., Khasanova S.R., Kudashkina N.V. et al. Selecting the optimal terms of extraction antocian compounds from the fruit of the hawthorn soft. Vestnik Bashkirskogo gosudarstvennogo meditsinskogo universiteta. 2018; 4: 130–3 (in Russian).
  3. Morozova T.V., Kurkin V.A., Pravdivtseva O.E. et al. The content of total flavonoids in the fruits and shoots of some hawthorn species. Aspirantskiy vestnik Povolzh'ya. 2018; 1–2: 22–4 (in Russian).
  4. Morozova T.V., Kurkin V.A., Zaitceva E.N. et al. Study of antidepressant properties of the liquid extacts based on raw materials of Crataegus submollis SARG. Vestnik Bashkirskogo gosudarstvennogo meditsinskogo universiteta. 2018; 4: 150–5 (in Russian).
  5. The State Pharmacopoeia of the Russian Federation. XIV edition. [Electronic resource]. Access mode: http://femb.ru/feml (In Russian).
  6. Kurkina A.V. Flavonoids of pharmacopoeial plants. Samara: Ofort, SamGMU, 2012; 290 (In Russian).
  7. Kurkina A.V. New approaches of standardization of hawthorn flowers. Khimiya rastitel'nogo syr'ya. 2013; 2: 171–6 (In Russian)