Procedure for quantitative determination of the amount of flavonoids in pedunculate oak (Quercus robur) leaves

DOI: https://doi.org/10.29296/25419218-2021-02-04
Issue: 
2
Year: 
2021

N.A. Ryabov, V.M. Ryzhov Samara State Medical University, 89, Chapaevskaya St., Samara 443099, Russian Federation

Introduction. Pedunculate oak (Quercus robur L.), a member of the beech (Fagaceae) family, is a medicinal plant. Young tree bark that has astringent and anti-inflammatory properties due to tannins present in the raw material is used as raw material. Other plant organs, oak leaves in particular, are also of interest. The preliminary phytochemical study has shown that they contain flavonoid compounds in addition to tannins. Objective: to develop a procedure for quantitative determination of the amount of flavonoids in the pedunculate oak leaves. Material and methods. The investigation object was pedunculate oak leaves harvested in the Pokhvistnevsky District (Samara Region) in June-August 2019. Direct and differential spectrophotometry was used to develop the procedure. Results. The investigators determined optimal conditions for the quantitative determination of the amount of flavonoids in the pedunculate oak leaves: the extractant was 80% ethyl alcohol; the raw material-extractant ratio was 1:50; the extraction time was 30 minutes, the degree of raw material grinding was 2 mm. Validation studies revealed the validity of the developed procedure. Conclusion. The procedure has been developed for the quantitative determination of the amount of flavonoids in the pedunculate oak leaves calculated with reference to rutin, by using the differential spectrophotometric method at an analytical wavelength of 412 nm. The total flavonoid content in the pedunculate oak leaves ranges from 0.30 to 1.63%.

Keywords: 
pedunculate oak
Quercus robur L.
leaves
standardization
flavonoids
rutin
spectrophotometry

References: 
  1. The State Pharmacopoeia оf the Russian Federation, XIV-ed. [Electronic resource]. Access mode: http://femb.ru/femb/pharmacopea.php (in Russian).
  2. Karomatov I.D., Makhmudova G.F. Kesey. Quercus robur – use in medical practice. Biologiya I intergal'naya meditsina. 2016; 3: 41–7 (in Russian).
  3. Kiseleva T.L., Smirnova Yu.A. Medicinal plants in world medical practice: state regulation of the nomenclature and quality. Moscow: Izdatel'stvo professional'noy assotsiatsii naturoterapevtov, 2009; 183–4 (in Russian).
  4. Kurkin VA. Pharmacognosy. Samara: «Ofort», 2019; 966–9 (in Russian).
  5. Plant resources of Russia: Wild flowering plants, their component, composition and biological activity. Vol. 2. Families of Actinidiaceae-Malvaceae, Euphorbiaceae-Haloragaceae. Saint-Petersburg, Moscow: Tovarishchestvo nauchnykh izdaniy KMK, 2009; 513 (in Russian).
  6. Kurkina AV. Flavonoids of pharmacopeia plants. Samara: SamGMU, «Ofort», 2012; 290 (in Russian).
  7. Kupriyanova E.A., Kurkin V.A. Development of approaches to the standardization of black poplar leaves. Аспирантский вестник Поволжья. 2018; 5 (6): 17–21. DOI: 10.17816/2072-2354.2018.18.3.17-21 (in Russian).