The anatomical and morphological characteristics of the leaves of common myrtle (Myrtus commonis) growing under the conditions of the Southern Coast of the Crimea

DOI: https://doi.org/10.29296/25419218-2021-02-05
Issue: 
2
Year: 
2021

E.Yu. Bakova(1), F.M. Melikov(1), D.A. Konovalov(2), N.V. Bobkova(3) 1-Nikita Botanical Garden, National Research Center, Russian Academy of Sciences, Nikita Urban-Type Settlement, Yalta 298648, Republic of Crimea, Russian Federation; 2-Pyatigorsk Medical Pharmaceutical Institute, Branch, Volgograd State Medical University, 11, Kalinin Prospect, Pyatigorsk 357532, Russian Federation; 3-I.M. Sechenov First Moscow State Medical University (Sechenov University); 8, Trubetskaya St., Build. 2, Moscow 119048, Russia

Introduction. Common myrtle (Myrtus commonis L.) is a promising medicinal plant that is comprehensively being studied in the Nikita Botanical Garden to be introduced into industrial culture as a raw source for obtaining medicinal plant raw materials. Objective. To investigate the morphological and anatomical characteristics of myrtle leaves as characteristics of the identity of promising medicinal plant raw materials. Material and methods. The investigation object was fresh and dried whole and crushed leaves of common myrtle cultivated at the production site of the Nikita Botanical Garden (the Republic of Crimea). The myrtle leaves underwent morphological and anatomical studies in accordance with the general pharmacopoeia articles «Leaves» and «Technique for microscopic and microchemical studies of medicinal plant raw materials and herbal medicines» in the 14th Edition of the Russian Federation’s State Pharmacopoeia, by using transmitted or polarized light microscopy. Results. The external signs of the whole leaves of common myrtle, the characteristic features of which may include the shape and size of the leaves and the characteristic taste and odor of the raw material, were determined. The anatomical and diagnostic signs of the whole leaves and their powder (the dorsoventral structure of the leaf blade; the presence of anomocytic stomata only on the abaxial side; conceptacles with essential oil; druses and prismatic crystals of calcium oxalate) were revealed. The biometric anatomical and diagnostic characteristics were determined. Conclusion. The morphological and anatomical characteristics of the whole and crushed leaves of common leaves were established in accordance with the pharmacopoeial requirements.

Keywords: 
common myrtle
Myrtus communis L.
medicinal plant raw materials
external signs
microscopic analysis
polarizing light microscopy

References: 
  1. Mimica-Dukic N., Bugarin D., Grbovic S., Mitic-Culafic D., Vukovic-Gacic B., Orcic D., Jovin E., Couladis M.. Essential Oil of Myrtus communis L. as a Potential Antioxidant and Antimutagenic Agents. Molecules. 2010; 4: 2759–70. Available at: http://www.mdpi.com/1420-3049/15/4/2759?utm_source=TrendMD&utm_medium=cpc&utm_campaign=Molecules_TrendMD_1 (Accessed: 27.01.2021).
  2. Sabiha Sumbul, M. Aftab Ahmad, M. Asif, Mohd Akhtar, Ibne Saud. Physicochemical and phytochemical standardization of berries of Myrtus communis Linn. J. of pharmacy & bioallied sciences. 2012; 4 (4): 322. Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/pmc3523529/ (Accessed: 11.02.2021).
  3. Eslami, Sh.; Mozdastan, Sh.; Ebrahimzadeh M.A. Antioxidant activity of polyphenol and flavonoid rich extracts from leaves of Myrtle (Myrtus communis L). Pharmacologyonline. 2016; 2: 132–6. Available at: https://www.researchgate.net/profile/Mohammad_Ebrahimzadeh/publication/308161250_Antioxidant_activity_of_polyphenol_and_flavonoid_rich_extracts_from_leaves_of_myrtle_Myrtus_communis_L/links/57de165b08ae4e6f1849ad68.pdf Accessed: 18.12.2020)
  4. Mozdastan S., Ebrahimzadeh M. A., Eslami S. Effect of increasing the polarity of solvent on total phenol and flavonoid contents and antioxidant activity of Myrtle (Myrtus communis L.). J. of Mazandaran University of Medical Sciences. 2015; 25 (126): 68–81. Available at: http://jmums.mazums.ac.ir/files/site1/user_files_0d0bf0/malavi-A-10-4333-70-157fca1.pdf (Accessed: 11.02.2021).
  5. Wannes W.A, Mhamdi B., Sriti J., Jemia M. B., Ouchikh O., Hamdaoui G., Kchouk M. E., Marzouk B. Antioxidant activities of the essential oils and methanol extracts from myrtle (Myrtus communis var. italica L.) leaf, stem and flower. Food and Chemical Toxicology. 2010; 5: 1362–70. Available at: http://www.sciencedirect.com/science/article/pii/S0278691510001511 (Accessed: 11.02.2021).
  6. Plugatar' Ju. V., Shevchuk O. M. Itogi i napravlenija selektsii aromaticheskih i lekarstvennyh rastenij v Nikitskom botanicheskom sadu. Bjulleten' Gosudarstvennogo Nikitskogo botanicheskogo sada. 2019; 130. URL: https://cyberleninka.ru/article/n/itogi-i-napravleniya-selektsii-aromaticheskih-i-lekarstvennyh-rasteniy-v-nikitskom-botanicheskom-sadu/viewer (data obraschenija: 25.12.2020). [Plugatar Yu. V., Shevchuk O. M. The results and directions of aromatic and medicinal plants’ selection in the Nikitsky Botanical Gardens. Bulletin of the State Nikitsky Botanical Gardens. 2019; 130. URL: https://cyberleninka.ru/article/n/itogi-i-napravleniya-selektsii-aromaticheskih-i-lekarstvennyh-rasteniy-v-nikitskom-botanicheskom-sadu/viewer (date of access: 25.12.2020) (in Russian)].
  7. Logvinenko L.A. Osobennosti biologii rosta i razvitija mnogoletnej i poroslevoj formy mirta obyknovennogo (Myrtus communis L.) v uslovijah Juzhnogo berega Kryma. Ural'skij agrarnyj vestnik. 2017; 11 (165): 45–9. [Logvinenko L.A. Osobennosti biologii rosta i razvitija mnogoletnej i poroslevoj formy mirta obyknovennogo (Myrtus communis L.) v uslovijah Juzhnogo berega Kryma. Ural'skij agrarnyj vestnik. 2017; 11 (165): 45–9 (in Russian)].
  8. Mastrogiovanni F., Ovidi E., Mamadalieva N.Z., Tiezzi A.. Testings of Myrtus communis leaf extracts on mammalian and bacteria cells. New Aspects in Medicinal Plants and Pharmacognosy. 2017; 147–59. Available at: http://books.tmkarpinski.com/9-TiezziKarpinski-2017.pdf (Accessed: 21.12.2020).
  9. Rabotjagov V.D., Hlypenko L.A., Bakova N.N., Mashanov V.I. Annotirovannyj katalog vidov i sortov efiromaslichnyh, prjano-aromaticheskih i pischevyh rastenij kollektsii Nikitskogo botanicheskogo sada. Nikitskij botanicheskij sad-Natsional'nyj nauchnyj tsentr. Jalta: NBS-NNTs, 2007; 48. [Rabotjagov V.D., Hlypenko L.A., Bakova N.N., Mashanov V.I. Annotirovannyj katalog vidov i sortov jefiromaslichnyh, prjano-aromaticheskih i pishhevyh rastenij kollekcii Nikitskogo botanicheskogo sada. Nikitskij botanicheskij sad-Nacional'nyj nauchnyj centr. Jalta: NBS-NNC, 2007; 48 (in Russian)].
  10. Cherjatova Ju.S. Sekretornye struktury list'ev Mirta obyknovennogo (Myrtus communis L.). Nauka segodnja: problemy i perspektivy razvitija: sb. nauch. tr. Nauchnyj tsentr «Disput». Vologda, 2015; 107–8. [Cherjatova Ju.S. Sekretornye struktury list'ev Mirta obyknovennogo (Myrtus communis L.). Nauka segodnja: problemy i perspektivy razvitija: sb. nauch. tr. /Nauchnyj centr «Disput». Vologda, 2015; 107–8 (in Russian)].
  11. Gosudarstvennaja farmakopeja RF HIV izd.: http://resource.rucml.ru/feml/pharmacopia/14_2/HTML/517/index.html (data obraschenija 18.02.2021) [Gosudarstvennaya farmakopeya RF HIV izd.: http://resource.rucml.ru/feml/pharmacopia/14_2/HTML/517/index.html (data obrashcheniya 18.02.2021) (in Russian)].
  12. Kurkin V.A., Avdeeva E.V., Tarasenko L.V. Ryzhov V.M., Shagalieva N.R., Aenagulova A.V., Marlynova L.V. Sravnitel'noe anatomo-morfologicheskoe issledovanie nekotoryh vegetativnyh organov evkalipta prutovidnogo i evkalipta serogo. Meditsinskij al'manah. 2013; 5 (29): 191–6. [Kurkin V.A., Avdeeva E.V., Tarasenko L.V. Ryzhov V.M., Shagalieva N.R., Ajenagulova A.V., Marlynova L.V. Sravnitel'noe anatomo-morfologicheskoe issledovanie nekotoryh vegetativnyh organov jevkalipta prutovidnogo i jevkalipta serogo. Medicinskij al'manah. 2013; 5 (29): 191–6 (in Russian)].