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Evaluation of the wound-healing effect of an N-isopropenylimidazole zinc metal complex derivative on a linear skin wound model in rats


S.A. Lebedeva(1), P.A. Galenko-Yaroshevsky (Jr.(2) , S.I. Melnik(1), S.V. Kozin(1), T.A. Demura(1), J.R. Arshinov(1), O.N. Gulevskaya(3), P.A. Galenko-Yaroshevsky(3) 1-I.M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Health of Russia, 8, Trubetskaya St., Build. 2, Moscow, 119991, Russian Federation; 2-State Budgetary Health Care Institution «Leningrad Central District Hospital» of the Ministry of Health of the Krasnodar Territory, 302 Divisii str., 24, Leningradskaya stanitsa, Leningradsky district, Krasnodar Territory, 353740, Russian Federation; 3-Kuban State Medical University, 4, Mitrofan Sedin St., Krasnodar, 350063, Russian Federation

Introduction. To search for drugs that have a pronounced wound-healing effect is one of the urgent tasks of modern pharmacology and medicine in general. The essential elements, among which zinc is of particular interest, play an important role in the multicomponent cascade mechanism of wound healing. It has been presently proven that this trace element has anti-inflammatory, antibacterial, membrane-stabilizing, and antioxidant activities, and is also an indispensable participant in the processes of keratinocyte migration, proliferation, and differentiation. Objective: to evaluate the wound-healing effect of 1% N-isopropenylimidazole zinc organometallic complex gel on the course of a non-infected wound process when modeling a linear skin wound in rats. Material and methods. The investigation object was 1% (N-isopropenylimidazole) zinc diacetate gel (Pilim-1) based on sodium carboxymethylcellulose (NaCMC). 10% methyluracil ointment for topical application and 0.5% zinc sulfate gel based on NaCMC were selected as reference agents. The linear skin wound model was reproduced on anesthetized rats. The course of a wound process was assessed by the rate of wound epithelialization, as well as by the nature of granulation tissue healing, repair, and maturity, the degree of angiogenesis, the presence of a purulent process and other criteria identified while histologically examining the wound skin samples. Results. The morphological and anatomical signs of the leaves of Gledichia vulgaris have been established. The main diagnostic microscopic signs are: the anomocytic type of the stomatal apparatus; simple single-celled thick-walled simple hairs with a folded cuticle on the upper side of the epidermis; the presence of druses in the mesophyll, the largest number of which is located in the central part of the plate; crystal-bearing lining of leaf veins; the presence of clusters of extractive substances in mesophyll cells; reduced denticles along the edge of the leaf plate. Conclusion. The findings suggest that there are prospects for designing 1% gel Pilim-1 as an effective wound healing agent.

linear wound
N-isopropenylimidazole derivative
wound healing
reparative regeneration

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