Evaluation of the quality and biological activity of the «BioChaga» birch fungus extract

DOI: https://doi.org/10.29296/25419218-2022-02-06
Issue: 
2
Year: 
2022

O.N. Usoltseva(1), D.N. Olennikov(2), T.V. Potupchik(3) 1-Limited Liability Company «Sibpribor», St. Trilissera, 87, Irkutsk, 664047, Russian Federation; 2-Federal state budgetary institution of science of the Buryat scientific center, Siberian branch of the Russian Academy of Sciences, street Casanovas, 8, Ulan-Ude, 670047, Russian Federation; 3-Federal state budgetary educational institution of higher education «Krasnoyarsk state medical University named after Professor V.F. Voyno-Yasenetsky» of the Ministry of Health of the Russian Federation, Partizan Zheleznyak str., 1, Krasnoyarsk, 660022, Russian Federation

Introduction. «BioСhaga» is a natural extract of the birch mushroom chaga, produced according to the author's technology of the Irkutsk company SibPribor LLC under the trademark «Baikal Legend». The high quality of «BioСhaga» is due to the combination of environmentally friendly chaga medicinal plant raw materials rich in biologically active substances of the Baikal region and the unique author's technology of its processing. The result is a fast-soluble «BioСhaga» powder with low density, high biological activity and bioavailability. The purpose of the study: a comprehensive study of the composition and content of BAS, biological activity of the «BioСhaga» extract in connection with the peculiarities of its production technology. Material and methods. To determine the physicochemical properties of «BioСhaga» extract we used the following methods: extraction / gravimetry, spectrophotometry, the qualitative composition was studied using high-performance liquid chromatography. To determine the biological activity of «BioСhaga», the antiradical activity of the extract to organic and inorganic radicals of natural and artificial origin was determined by spectrometry and coulometry. The antioxidant potential was evaluated using spectrophotometry and fluorimetry to determine the total antioxidant potential and the ability to absorb oxygen radicals. The iron-reducing and iron-chelating potential of «BioСhaga» was determined by spectrophotometry. Results. The physico-chemical characteristics of the «BioСhaga» composition had a high level of compounds characteristic of the chaga: low molecular weight phenolic compounds – 57,00±1,48 mg/kg, high molecular weight phenolic compounds (melanin complex) – 24,9±1,2%. The nature of the distribution of fragments of the melanin complex shows a predominant content of o-dioxybenzoyl fragments, which suggests the presence of high biological activity in the «BioСhaga». It has been shown that the «BioСhaga» has antioxidant, antiradical and iron-reducing activity. In addition, the extract has proven to be a good inactivator of nitric oxide molecules, which indicates its anti-inflammatory properties. Conclusion. «BioСhaga» is a highly active natural complex agent characterized as an effective antioxidant created using a unique author's technology. Its high biological activity indicates the effectiveness of the extract and allows it to be recommended as a means of prevention and protection against oxidative stress.
Keywords: 
«BioChaga»
birch mushroom chaga
Inonotus obliquus
production technology
biologically active substance
biologically active additive
References: 
  1. Strupan E.A., Kolodjaznaja V.S., Strupan O.A Tehnologija poluchenija ekstraktov iz dikorastuschego rastitel'nogo syr'ja, shiroko primenjaemogo v pischevoj promyshlennosti i fitoterapii. Vestnik KrasGAU. 2012; 8: 199–205. [Strupan E.A., Kolodyaznaya V.S., Strupan O.A. Technology for obtaining extracts from wild plant raw materials widely used in the food industry and phytotherapy. Bulletin of KrasGAU. 2012; 8: 199–205 (in Russian)].
  2. Hasanov V.V., Slizhov Ju.G., Chumakov A.A., Bazhina S.V «Analiz sostava i svojstv syr'ja berezovogo griba chagi Inonotus obliquus (PERS.) PILAT, sobrannogo v permskoj oblasti. Himija rastitel'nogo syr'ja. 2015; 2: 43–8. [Khasanov V.V., Slizhov Yu.G., Chumakov A.A., Bazhina S.V. Analysis of the composition and properties of raw materials of the birch fungus chaga Inonotus obliquus (PERS.) PILAT collected in the Perm region. Chemistry of plant raw materials. 2015; 2: 43–8 (in Russian)]. DOI: 10.14258/jcprm.201502533
  3. Salova T.Ju., Gromova N.Ju. Teoreticheskie aspekty poluchenija biologicheski aktivnyh veschestv iz rastitel'nogo i zhivotnogo syr'ja. Uspehi sovremennogo estestvoznanija. 2016; 3: 39–43. [Salova T.Yu., Gromova N.Yu. Theoretical aspects of obtaining biologically active substances from plant and animal raw materials. The successes of modern natural science. 2016; 3: 39–43 (in Russian)].
  4. Balandajkin M.E. K voprosu ob izuchenii himicheskoj struktury i lechebnyh svojstv Inonotus obliquus (PERS.) Pil. Himija rastitel'nogo syr'ja. 2013; 2: 15–22. [Balandaykin M.E. On the study of the chemical structure and medicinal properties of Inonotus obliquus (PERS.) Chemistry of plant raw materials. 2013; 2: 15–22. (in Russian)]. DOI: 10.14258/jcprm.1302015
  5. Perevedentseva L. Ispol'zovanie dikorastuschih gribov v lechebnyh tseljah v Permskom krae, Rossija. Ekologija i inzhenerija. 2013; 2: 236–42. [Perevedentseva L. The use of wild mushrooms for medicinal purposes in the Perm Region, Russia. Ecology and engineering. 2013; 2: 236–42 (in Russian)].
  6. Zmitrovich I.V., Denisova N.P., Balandajkin M.E. i dr. Chaga i ee bioaktivnye kompleksy: istorija i perspektivy. Formuly farmatsii. 2020; 2 (2): 84–93. [Zmitrovich I.V., Denisova N.P., Balandaykin M.E. et al. Chaga and its bioactive complexes: history and prospects. Pharmacy formulas. 2020; 2 (2): 84–93 (in Russian)]. DOI: 10.17816/phf34803
  7. Ma L., Cen H., Dong P., Lu H. et al. Anti-inflammatory and antitumor activity of extracts and compounds from the fungus Inonotus obliquus. Food Chem. 2013; 139 (1–4): 503–8. DOI: 10.1016/j.foodchem.2013.01.030
  8. Semenova E.V., Tjumentseva V.R., Kozubenko A.A. i dr. Biologicheski aktivnye soedinenija gribov – istochnik innovatsij v meditsine. Sovremennye problemy nauki i obrazovanija. 2020; 1. Dostupno na: https://science-education.ru/ru/article/view?id=29455. [Semenova E.V., Tyumentseva V.R., Kozubenko A.A. et al. Biologically active compounds of fungi are a source of innovation in medicine. Modern problems of science and education. 2020; 1. Available at: https://science-education.ru/ru/article/view?id=29455 (in Russian)].
  9. Sysoeva M.A, Habibrahmanova V.R., Gamajurova V.S. i dr. Issledovanie zolja vodnyh izvlechenij chagi. XII. Osazhdenie disperstnoj fazy vodnogo izvlechenija chagi pri izmenenii rN sredy. Himija rastitel'nogo syr'ja. 2009; 1: 131–5. [Sysoeva M.A., Khabibrakhmanova V.R., Gamayurova V.S. et al. Investigation of sol of aqueous extracts of chaga. XII. Precipitation of the dispersed phase of aqueous extraction of chaga with a change in the pH of the medium. Chemistry of plant raw materials. 2009; 1: 131–5 (in Russian)].
  10. Živković L., Bajić V., Topalović D. et al. Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells. Oxid Med Cell Longev. 2019; 2019: 5039372. DOI: 10.1155/2019/5039372.
  11. Olennikov D., Tankhaeva L.M., Rokhin A.V. et al. Physicochemical properties and antioxidant activity of melanin fractions from Inonotus obliquus sclerotia. Chemistry of Natural Compounds. 2012; 48 (3). DOI:10.1007/s10600-012-0260-y
  12. Gosudarstvennyj reestr lekarstvennyh sredstv. https://grls.rosminzdrav.ru/GRLS.aspx [State Register of Medicines. https://grls.rosminzdrav.ru/GRLS.aspx (in Russian)].
  13. Gosudarstvennaja Farmakopeja Rossijskoj Federatsii. XIV izdanie. Moskva, 2018. [The State Pharmacopoeia of the Russian Federation. XIV edition Moscow, 2018. (in Russian)].
  14. Prieto P., Pineda M., Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem. 1999; 269 (2): 337–41. DOI: 10.1006/abio.1999.4019.
  15. Seyoum, A., Asres, K., El-Fiky, F.K. Structure-Radical Scavenging Activity Relationships of Flavonoids. Phytochemistry, 2006; 67: 2058–70. http://dx.doi.org/10.1016/j.phytochem.2006.07.002
  16. Yen G.-C., Su H.-J., Yeh C.-T., Wu C.-H., Duh P.-D. scavenging effects of lotus seed extracts on reactive nitrogen species. 2006; 94 (4): 596–602 DOI: 10.1016/j.foodchem.2004.11.052
  17. Potapovich M.V., Metelitza D.I., Shadyro O.I., Kurchenko V.P. Antioxidant activity of oxygen-containing aromatic compounds. Appl. Biochem. Microbiol. 2011; 47 (4): 346–55. DOI: 10.1134/S0003683811040144
  18. Zhou B., Guo Z., Xing J., Huang B. Nitric oxide is involved in abscisic acid-induced antioxidant activities in Stylosanthes guianensis. Journal of Experimental Botany. 2005; 56: 3223–8.
  19. Song F.Q., Liu Y., Kong X.S. et al. Progress on understanding the anticancer mechanisms of medicinal mushroom: Inonotus obliquus. Asian Pac J. Cancer Prev. 2013; 14 (3): 1571–8. DOI: 10.7314/apjcp.2013.14.3.1571