Sorption technology for the isolation of pancreatic enzymes from extracts of the pancreas of cattle and reindeer

DOI: https://doi.org/10.29296/25419218-2023-05-05
Issue: 
5
Year: 
2023

L.I. Karavaeva(1), P.A. Krylova(1), A.S. Moroz (Kurochkina)(3), N.V. Kotova(1), N.V. Glazova(1), N.D. Bunyatyan(2)
1-St. Petersburg Chemical and Pharmaceutical University, Professor Popov str., 14, Sant-Petersburg, 197022, Russian Federation;
2-Federal State Budgetary Institution "Scientific Centre for Expert Evaluation of Medicinal Products" of the Ministry of Health of the Russian Federation, Petrovsky boulevard, 8/2, Moscow, 127051, Russian Federation;
3-Peter the Great St. Petersburg Polytechnic University, Politechnicheskaya str., 29, Saint-Petersburg, 195251, Russian Federation

Introduction. Pancreatin is a digestive enzyme remedy that is an extract of a pancreatic enzyme. It contains such proteolytic enzymes as amylase, lipase and protease, which are involved in the digestion of carbohydrates, fats, proteins, which contributes to their more complete absorption in the small intestine. It is used for insufficiency of the exocrine function of the pancreas (chronic pancreatitis, cystic fibrosis), for chronic inflammatory and degenerative diseases of the stomach, intestines, liver, gallbladder; conditions after resection or irradiation of these organs, accompanied by disorders of food digestion, flatulence, diarrhea (as part of combination therapy), to improve food digestion in patients with normal function of the gastrointestinal tract (GIT) in case of nutritional errors, as well as chewing disorders function, forced prolonged immobilization, sedentary lifestyle, and in preparation for x-ray and ultrasound examination of the abdominal organs.Thus, pancreatin is one of the most popular medical preparations and is produced under various trade names. However, pancreatin is not produced in Russia and is supplied to the country mainly from China and Germany. Objective: Development of a technology for the isolation of pacreatic enzymes from extracts of the semigastric glands of SO and cattle. Material and methods. In the work used – the pancreas from the reindeer. The determination of the component composition was carried out by the methods of gel chromatography. Results. Methods have been developed for isolating a complex of digestive enzymes from the pancreas of CO and cattle. Conclusion. The optimal conditions for the process of BAS extraction from the pancreas of CO and the pancreas of cattle have been selected. The component composition of the obtained extracts was determined.The optimization of the sorption technology for the isolation of enzymes from extracts of cattle and CO was developed and carried out.
Keywords: 
pancreas
reindeer
cattle
hydrolytic enzymes
sorption
desorption
frontal chromatography
stabilization
cyclodextrins.
References: 
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