Study of the component composition of peppermint leaves by HPLC method

DOI: https://doi.org/10.29296/25419218-2023-08-05
Issue: 
8
Year: 
2023

M.A. Kazakova, V.A. Kurkin, A.R. Mubinov
Federal State Budgetary Educational Institution of Higher Education «Samara State Medical University» of the Ministry of Healthcare of the Russian Federation, ul. Chapaevskaya, 89, Samara, 443099, Russian Federation

Introduction. Peppermint (Mentha piperita L.) is a medicinal plant widely cultivated in the Russian Federation and CIS countries. Peppermint contains various groups of biologically active substances (essential oil, flavonoids, phenylpropanoids), which allow this plant to be used in the medicine, and also in pharmaceutical, food, agricultural industries. The purpose of the study was to study the component composition of aqueous-alcoholic extracts from peppermint (Mentha piperita L.) leaves using HPLC. Material and methods. The research material was samples of peppermint leaves (JSC Krasnogorskleksredstva, Reg. No. LP-003986, as well as cultivated in the Botanical Garden of Samara University, Samara). Analysis of aqueous-alcoholic extracts from peppermint leaves by HPLC was carried out using working standard samples of rosmarinic acid, chlorogenic acid, caffeic acid, luteolin, cynaroside and 5,3'-dihydroxy-6,7,8,4'-tetramethoxyflavone, isolated by us preparatively. HPLC analysis is carried out under reversed-phase chromatography conditions in a gradient mode on a high-performance liquid chromatograph microcolumn liquid chromatograph "Milichrome-6" (NPJSC "Nauchpribor") in a gradient mode, steel column "KAH-6-80-4" (No. 2; 2×80 mm; Separon-C18 7 µm), eluent system: acetonitrile – 1% acetic acid solution, elution rate – 100 µl/min. Detection of substances was carried out at wavelengths of 290, 330 and 350 nm. The volume of injected samples is 2 µl. Results. It was determined that the dominant phenylpropanoid in the aqueous-alcoholic extract of peppermint leaves is rosmarinic acid, the retention time of which is 12,61 minutes. Chlorogenic acid, caffeic acid, cynaroside, luteolin, and 5,3'-dihydroxy-6,7,8,4'-tetramethoxyflavone were found and identified in the raw materials of this plant. Conclusion. As a result of the study it was determined that the dominant component of aqueous-alcoholic extracts from peppermint leaves is rosmarinic acid.
Keywords: 
peppermint; Mentha piperita; leaves; phenylpropanoids
rosmarinic acid
flavonoids
HPLC.
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