Study of the component composition of peppermint leaves by HPLC method


M.A. Kazakova, V.A. Kurkin, A.R. Mubinov
Federal State Budgetary Educational Institution of Higher Education «Samara State Medical University» of the Ministry of Healthcare of the Russian Federation, ul. Chapaevskaya, 89, Samara, 443099, Russian Federation

Introduction. Peppermint (Mentha piperita L.) is a medicinal plant widely cultivated in the Russian Federation and CIS countries. Peppermint contains various groups of biologically active substances (essential oil, flavonoids, phenylpropanoids), which allow this plant to be used in the medicine, and also in pharmaceutical, food, agricultural industries. The purpose of the study was to study the component composition of aqueous-alcoholic extracts from peppermint (Mentha piperita L.) leaves using HPLC. Material and methods. The research material was samples of peppermint leaves (JSC Krasnogorskleksredstva, Reg. No. LP-003986, as well as cultivated in the Botanical Garden of Samara University, Samara). Analysis of aqueous-alcoholic extracts from peppermint leaves by HPLC was carried out using working standard samples of rosmarinic acid, chlorogenic acid, caffeic acid, luteolin, cynaroside and 5,3'-dihydroxy-6,7,8,4'-tetramethoxyflavone, isolated by us preparatively. HPLC analysis is carried out under reversed-phase chromatography conditions in a gradient mode on a high-performance liquid chromatograph microcolumn liquid chromatograph "Milichrome-6" (NPJSC "Nauchpribor") in a gradient mode, steel column "KAH-6-80-4" (No. 2; 2×80 mm; Separon-C18 7 µm), eluent system: acetonitrile – 1% acetic acid solution, elution rate – 100 µl/min. Detection of substances was carried out at wavelengths of 290, 330 and 350 nm. The volume of injected samples is 2 µl. Results. It was determined that the dominant phenylpropanoid in the aqueous-alcoholic extract of peppermint leaves is rosmarinic acid, the retention time of which is 12,61 minutes. Chlorogenic acid, caffeic acid, cynaroside, luteolin, and 5,3'-dihydroxy-6,7,8,4'-tetramethoxyflavone were found and identified in the raw materials of this plant. Conclusion. As a result of the study it was determined that the dominant component of aqueous-alcoholic extracts from peppermint leaves is rosmarinic acid.

peppermint; Mentha piperita; leaves; phenylpropanoids
rosmarinic acid

  1. Kurkin V.A. Pharmacognosy: a textbook for students of pharmaceutical universities (faculties), 5th ed. reworked and additional – Samara: LLC “Printing Association “Standard””, Federal State Budgetary Educational Institution of Higher Education SamSMU of the Ministry of Health of Russia, 2020; 1278 (in Russian).
  2. Vidal’s reference book “Medicines in Russia” URL: (in Russian).
  3. Belyavskaya I.G., Alekseenko E.V., Kapustina K.F., Isabaev I.B. Development of technology for bakery products using peppercorn. Health, Food & Biotechnology, 2019; 1 (4): 53–69. DOI: 10.36107/hfb.2019.i4.s276 (in Russian).
  4. State Pharmacopoeia of the Russian Federation. XIV ed., Ministry of Health of the Russian Federation, 2018. URL: (in Russian).
  5. European Pharmacopoeia VIII edition, Peppermint leaf 01/2011:0406 (in Russian).
  6. European Pharmacopoeia VIII, edition Peppermint leaf dry extract 04/2009:2382 (in Russian).
  7. State Pharmacopoeia of the Republic of Belarus. Unitary Enterprise "Center for Expertise and Testing in Healthcare"; under general ed. A.A. Sheryakova., Minsk: Minsk State PTC of Printing named after. V. Khoruzhey, 2009 (in Russian).
  8. State Pharmacopoeia of the Republic of Kazakhstan. T. 2. Almaty: Publishing house “Zhibek Zholy”, 2009; 804 (in Russian).
  9. Kazakova M.A., Kurkin V.A. Current problems of standardization of peppermint. Current problems and prospects for pharmaceutical science and practice: materials of the III International Scientific and Practical Conference (Kemerovo, May 26, 2023). Collection. 2023; 259–64 (in Russian).
  10. Gudzenko A. Development and validation of a method for the determination of rosmarinic acid in Mentha piperita L. using solid-phase extraction and RP-HPLC with photodiode array detection. J. of Chemical and Pharmaceutical Research. 2013; 5 (9): 40–5.
  11. Jullien F., Voirin B., Bernillon J., Favre-Bonvin J. Highly oxygenated flavonoids from Mentha piperita. Phytochemistry. 1984; 23 (12): 2972–3.